產品詳情介紹
ProteinIso? Ni-NTA Resin是螯合金屬Ni2+而形成的一種親和層析介質。NTA能夠通過四個位點牢固地螯合Ni2+，從而減少純化過程中Ni2+泄漏到蛋白樣品中。本產品對His標簽蛋白具有特異吸附能力，從而使His標簽蛋白結合在Ni-NTA純化介質上，未結合的蛋白被洗滌下去。結合在介質上的蛋白經過一定濃度的咪唑或者pH低緩沖液溫和洗脫下來，從而得到高純度的目的蛋白。本產品具有吸附容量大、選擇性好、通透性強、易于再生等優(yōu)點，適用于在非變性或變性條件下純化任何表達系統表達的His標簽重組蛋白。
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